Congrats to Richard on his recent publication!

PCR-free, Multiplexed Expression Profiling of microRNAs using Silicon Photonic Microring Resonators

R.M. Graybill, C.S. Para, R.C. Bailey Analytical Chemistry, 2016, ASAP

Abstract

We describe an approach for multiplexed microRNA analysis using silicon photonic microring resonators to detect cDNA reverse transcription products via a subsequent enzymatic signal enhancement strategy. Key to this method is a modified stem loop primer that facilitates downstream signal amplification via enzymatic turnover and improves the sensor signal 20-fold when compared to traditional stem loop primers. This approach facilitates targeted microRNA quantification in only 2.5 hours and without requiring target amplification via the polymerase chain reaction (PCR). Primers for 7 miRNA targets were orthogonally designed to avoid cross-hybridization between capture probes. This approach was applied to the detection of total RNA from human tissues and found to display differential expression profiles consistent with literature precedent. This development holds promise as an alternative to single-plex RT-qPCR methods and more expensive RNA-seq by offering a cost-effective method to analyze targeted miRNA panels in emerging diagnostic applications.

Bioanalytical Sensors 2016 Gordon Research Conference

Heather recently attended the Bioanlaytical Sensors Gordon Research Seminar and Conference in Newport, Rhode Island.  Her poster, Multiplexed Cytokine-Chemokine Profiling Using Microring Resonator Arrays to Diagnose Latent Tuberculosis Infection, was awarded the Royal Society of Chemistry Analyst Top Poster Prize (of 5).  Consequently, Heather presented a short talk in the Young Investigator Late Breaking Topic session to close out the GRC.

Biosensors 2016 Conference

Enrique recently attended the Biosensors 2016 Congress in Gothenburg, Sweden. His talk, Multiplexed detection of clinically-relevant biomarker panels using a silicon photonic biosensing platform: Application to Sepsis and Tuberculosis diagnostics, reported on the lab's recent efforts to transform the microring resonator array technology into a robust in vitro diagnostic platform.

Congrats to Quique on a successful meeting and presentation!

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Congrats to Dan on his recent publication!

Nanoporous Gold Membranes as Robust Constructs for Selectively Tunable Chemical Transport

Daniel A. McCurry, and Ryan C. Bailey J. Phys. Chem. C, Just Accepted Manuscript
DOI: 10.1021/acs.jpcc.6b02759

Abstract

Nanopores are promising structures for small volume separations, but often require complex topdown fabrication and are not easily (re)configurable to allow for dynamically tuned separations. Herein we report the first use of nanoporous gold (NPG) membranes as tunable, charge-based separation elements. NPG is fabricated into a robust network of interconnecting pores via simple solution de-alloying. We demonstrate that control over the selective permeation of small molecule analytes can be controlled via three different methods: 1) anion adsorption at the Au surface; 2) pH-tuning of self-assembled monolayer (SAM) charge; and 3) electrochemical oxidation of a redox-active SAM. This simple and versatile membrane system is promising as a dynamically-tunable element for small volume separations.